Modular control of l-tryptophan isotopic substitution via an efficient biosynthetic cascade.
Org Biomol Chem
; 18(22): 4189-4192, 2020 06 10.
Article
em En
| MEDLINE
| ID: mdl-32452506
ABSTRACT
Isotopologs are powerful tools for investigating biological systems. We report a biosynthetic-cascade synthesis of Trp isotopologs starting from indole, glycine, and formaldehyde using the enzymes l-threonine aldolase and an engineered ß-subunit of tryptophan synthase. This modular route to Trp isotopologs is simple and inexpensive, enabling facile access to these compounds.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Glicina Hidroximetiltransferase
/
Triptofano
/
Triptofano Sintase
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article