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TRIM72 promotes alveolar epithelial cell membrane repair and ameliorates lung fibrosis.
Cong, Xiaofei; Nagre, Nagaraja; Herrera, Jeremy; Pearson, Andrew C; Pepper, Ian; Morehouse, Robell; Ji, Hong-Long; Jiang, Dianhua; Hubmayr, Rolf D; Zhao, Xiaoli.
Afiliação
  • Cong X; Department of Physiological Sciences, Eastern Virginia Medical School, Norfolk, Virginia, USA.
  • Nagre N; Department of Physiological Sciences, Eastern Virginia Medical School, Norfolk, Virginia, USA. NagreN@evms.edu.
  • Herrera J; Department of Medicine, University of Minnesota, Minneapolis, MN, USA.
  • Pearson AC; Department of Physiological Sciences, Eastern Virginia Medical School, Norfolk, Virginia, USA.
  • Pepper I; Department of Physiological Sciences, Eastern Virginia Medical School, Norfolk, Virginia, USA.
  • Morehouse R; Department of Physiological Sciences, Eastern Virginia Medical School, Norfolk, Virginia, USA.
  • Ji HL; Texas Lung Injury Institute, The University of Texas Health Science Center at Tyler, Tyler, TX, USA.
  • Jiang D; Department of Medicine, Cedars Sinai Medical Center, Los Angeles, CA, USA.
  • Hubmayr RD; Division of Pulmonary and Critical Care Medicine, Mayo Clinic, Rochester, MN, USA.
  • Zhao X; Department of Physiological Sciences, Eastern Virginia Medical School, Norfolk, Virginia, USA. xiaoli.zhao@nih.gov.
Respir Res ; 21(1): 132, 2020 May 29.
Article em En | MEDLINE | ID: mdl-32471489
BACKGROUND: Chronic tissue injury was shown to induce progressive scarring in fibrotic diseases such as idiopathic pulmonary fibrosis (IPF), while an array of repair/regeneration and stress responses come to equilibrium to determine the outcome of injury at the organ level. In the lung, type I alveolar epithelial (ATI) cells constitute the epithelial barrier, while type II alveolar epithelial (ATII) cells play a pivotal role in regenerating the injured distal lungs. It had been demonstrated that eukaryotic cells possess repair machinery that can quickly patch the damaged plasma membrane after injury, and our previous studies discovered the membrane-mending role of Tripartite motif containing 72 (TRIM72) that expresses in a limited number of tissues including the lung. Nevertheless, the role of alveolar epithelial cell (AEC) repair in the pathogenesis of IPF has not been examined yet. METHOD: In this study, we tested the specific roles of TRIM72 in the repair of ATII cells and the development of lung fibrosis. The role of membrane repair was accessed by saponin assay on isolated primary ATII cells and rat ATII cell line. The anti-fibrotic potential of TRIM72 was tested with bleomycin-treated transgenic mice. RESULTS: We showed that TRIM72 was upregulated following various injuries and in human IPF lungs. However, TRIM72 expression in ATII cells of the IPF lungs had aberrant subcellular localization. In vitro studies showed that TRIM72 repairs membrane injury of immortalized and primary ATIIs, leading to inhibition of stress-induced p53 activation and reduction in cell apoptosis. In vivo studies demonstrated that TRIM72 protects the integrity of the alveolar epithelial layer and reduces lung fibrosis. CONCLUSION: Our results suggest that TRIM72 protects injured lungs and ameliorates fibrosis through promoting post-injury repair of AECs.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fibrose Pulmonar Idiopática / Células Epiteliais Alveolares / Proteínas com Motivo Tripartido / Pulmão Limite: Animals / Female / Humans / Male Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fibrose Pulmonar Idiopática / Células Epiteliais Alveolares / Proteínas com Motivo Tripartido / Pulmão Limite: Animals / Female / Humans / Male Idioma: En Ano de publicação: 2020 Tipo de documento: Article