Peptidyl ω-Asp Selenoesters Enable Efficient Synthesis of N-Linked Glycopeptides.
Front Chem
; 8: 396, 2020.
Article
em En
| MEDLINE
| ID: mdl-32478036
ABSTRACT
Chemical synthesis is an attractive approach allows for the assembly of homogeneous complex N-linked glycopeptides and glycoproteins, but the limited coupling efficiency between glycans and peptides hampered the synthesis and research in the related field. Herein we developed an alternative glycosylation to construct N-linked glycopeptide via efficient selenoester-assisted aminolysis, which employs the peptidyl ω-asparagine selenoester and unprotected glycosylamine to perform rapid amide-bond ligation. This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
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MEDLINE
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En
Ano de publicação:
2020
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Article