Biosynthesis of Alanyl-Histidine Dipeptide Catalyzed by Papain Immobilized on Magnetic Nanocrystalline Cellulose in Deep Eutectic Solvents.
Appl Biochem Biotechnol
; 192(2): 573-584, 2020 Oct.
Article
em En
| MEDLINE
| ID: mdl-32488610
ABSTRACT
Papain (PA) immobilized onto magnetic nanocrystalline cellulose (PA@MNCC) was successfully fabricated and adopted as an efficient biocatalyst for the synthesis of N-(benzyloxycarbonyl)-alanyl-histidine (Z-Ala-His) dipeptide. Introducing deep eutectic solvents (DESs) as reaction media promoted the synthesis of the Z-Ala-His dipeptide. The effects of reaction conditions on the yield of papain catalytic Z-Ala-His were systematically investigated with the highest yield of 68.4%, which was higher than free papain (63.3%). Besides, this novel PA@MNCC composite can be easily recycled from the reaction system by magnetic forces. In a word, the PA@MNCC composite exhibited great potential for efficient biosynthesis of dipeptide in DESs.
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Base de dados:
MEDLINE
Assunto principal:
Solventes
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Papaína
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Celulose
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Nanoestruturas
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Dipeptídeos
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Enzimas Imobilizadas
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Biocatálise
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article