Reproducibility-optimized detection of differential DNA methylation.
Epigenomics
; 12(9): 747-755, 2020 05.
Article
em En
| MEDLINE
| ID: mdl-32496849
ABSTRACT
Aim:
DNA methylation is a key epigenetic mechanism regulating gene expression. Identifying differentially methylated regions is integral to DNA methylation analysis and there is a need for robust tools reliably detecting regions with significant differences in their methylation status. Materials &methods:
We present here a reproducibility-optimized test statistic (ROTS) for detection of differential DNA methylation from high-throughput sequencing or array-based data.Results:
Using both simulated and real data, we demonstrate the ability of ROTS to identify differential methylation between sample groups.Conclusion:
Compared with state-of-the-art methods, ROTS shows competitive sensitivity and specificity in detecting consistently differentially methylated regions.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Análise de Sequência de DNA
/
Metilação de DNA
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article