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Direct imaging of rapid tethering of synaptic vesicles accompanying exocytosis at a fast central synapse.
Miki, Takafumi; Midorikawa, Mitsuharu; Sakaba, Takeshi.
Afiliação
  • Miki T; Graduate School of Brain Science, Doshisha University, 610-0394 Kyoto, Japan; tmiki@mail.doshisha.ac.jp tsakaba@mail.doshisha.ac.jp.
  • Midorikawa M; Organization for Research Initiatives and Development, Doshisha University, 610-0394 Kyoto, Japan.
  • Sakaba T; Division of Neurophysiology, Department of Physiology, School of Medicine, Tokyo Women's Medical University, 162-8666 Tokyo, Japan.
Proc Natl Acad Sci U S A ; 117(25): 14493-14502, 2020 06 23.
Article em En | MEDLINE | ID: mdl-32513685
ABSTRACT
A high rate of synaptic vesicle (SV) release is required at cerebellar mossy fiber terminals for rapid information processing. As the number of release sites is limited, fast SV reloading is necessary to achieve sustained release. However, rapid reloading has not been observed directly. Here, we visualize SV movements near presynaptic membrane using total internal reflection fluorescence (TIRF) microscopy. Upon stimulation, SVs appeared in the TIRF-field and became tethered to the presynaptic membrane with unexpectedly rapid time course, almost as fast as SVs disappeared due to release. However, such stimulus-induced tethering was abolished by inhibiting exocytosis, suggesting that the tethering is tightly coupled to preceding exocytosis. The newly tethered vesicles became fusion competent not immediately but only 300 ms to 400 ms after tethering. Together with model simulations, we propose that rapid tethering leads to an immediate filling of vacated spaces and release sites within <100 nm of the active zone by SVs, which serve as precursors of readily releasable vesicles, thereby shortening delays during sustained activity.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Vesículas Sinápticas / Cerebelo / Transmissão Sináptica / Modelos Neurológicos / Fibras Nervosas Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Vesículas Sinápticas / Cerebelo / Transmissão Sináptica / Modelos Neurológicos / Fibras Nervosas Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article