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Placental Metabolomics for Assessment of Sex-specific Differences in Fetal Development During Normal Gestation.
Saoi, Michelle; Kennedy, Katherine M; Gohir, Wajiha; Sloboda, Deborah M; Britz-McKibbin, Philip.
Afiliação
  • Saoi M; Department of Chemistry and Chemical Biology, McMaster University, Hamilton, Canada.
  • Kennedy KM; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Canada.
  • Gohir W; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Canada.
  • Sloboda DM; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Canada.
  • Britz-McKibbin P; Department of Pediatrics and Obstetrics and Gynecology, McMaster University, Hamilton, Canada.
Sci Rep ; 10(1): 9399, 2020 06 10.
Article em En | MEDLINE | ID: mdl-32523064
The placenta is a metabolically active interfacial organ that plays crucial roles in fetal nutrient delivery, gas exchange and waste removal reflecting dynamic maternal and fetal interactions during gestation. There is growing evidence that the sex of the placenta influences fetal responses to external stimuli in utero, such as changes in maternal nutrition and exposure to environmental stressors. However, the exact biochemical mechanisms associated with sex-specific metabolic adaptations during pregnancy and its link to placental function and fetal development remain poorly understood. Herein, multisegment injection-capillary electrophoresis-mass spectrometry is used as a high throughput metabolomics platform to characterize lyophilized placental tissue (~2 mg dried weight) from C57BL/6J mice fed a standardized diet. Over 130 authentic metabolites were consistently measured from placental extracts when using a nontargeted metabolomics workflow with stringent quality control and robust batch correction. Our work revealed distinct metabolic phenotype differences that exist between male (n = 14) and female (n = 14) placentae collected at embryonic day E18.5. Intracellular metabolites associated with fatty acid oxidation and purine degradation were found to be elevated in females as compared to male placentae (p < 0.05, effect size >0.40), including uric acid, valerylcarnitine, hexanoylcarnitine, and 3-hydroxyhexanolycarnitine. This murine model sheds new insights into sex-specific differences in placental mitochondrial function and protective mechanisms against deleterious oxidative stress that may impact fetal growth and birth outcomes later in life.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Placenta / Desenvolvimento Fetal / Metaboloma / Feto Tipo de estudo: Prognostic_studies Limite: Animals / Pregnancy Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Placenta / Desenvolvimento Fetal / Metaboloma / Feto Tipo de estudo: Prognostic_studies Limite: Animals / Pregnancy Idioma: En Ano de publicação: 2020 Tipo de documento: Article