PDGFRß plays an essential role in patient vitreous-stimulated contraction of retinal pigment epithelial cells from epiretinal membranes.
Exp Eye Res
; 197: 108116, 2020 08.
Article
em En
| MEDLINE
| ID: mdl-32561481
ABSTRACT
Platelet-derived growth factor (PDGF) is associated with clinical proliferative vitreoretinopathy (PVR), which is characterized by formation of sub- or epi-retinal membranes that consist of cells including retinal pigment epithelial ï¼RPEï¼ cells and extracellular matrix. RPE cells play an important role in PVR pathogenesis. Previous findings indicated that PDGF receptor (PDGFR)α was essential in experimental PVR induced by fibroblasts. In RPE cells derived from epiretinal membranes from patients with PVR (RPEMs)ï¼ Akt was activated by PDGF-B but not PDGF-A, which suggested that PDGFRß was the predominant PDGFR isoform expressed in RPEMs. Indeed, CRISPR/Cas9-mediated depletion of PDGFRß in RPEMs attenuated patient vitreous-induced Akt activation and cellular responses intrinsic to PVR including cell proliferation, migration, and contraction. We conclude that PDGFRß appears to be the PVR relevant PDGFR isoform in RPEMs.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA
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Regulação da Expressão Gênica
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Membrana Epirretiniana
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Receptor beta de Fator de Crescimento Derivado de Plaquetas
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Epitélio Pigmentado da Retina
Limite:
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article