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Development of novel EST microsatellite markers for genetic diversity analysis and correlation analysis of velvet antler growth characteristics in Sika deer.
Jia, Boyin; Wang, Guiwu; Zheng, Junjun; Yang, Wanyun; Chang, Shuzhuo; Zhang, Jiali; Liu, Yuan; Li, Qining; Ge, Chenxia; Chen, Guang; Liu, Dongdong; Yang, Fuhe.
Afiliação
  • Jia B; College of Animal Science and Technology, Jilin Agricultural University, 2888 Xincheng Street, Changchun, 130118, China.
  • Wang G; Institute of Wild Economic Animals and Plants and State Key Laboratory for Molecular Biology of Special Economical Animals, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun, 130112, China.
  • Zheng J; Institute of Wild Economic Animals and Plants and State Key Laboratory for Molecular Biology of Special Economical Animals, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun, 130112, China.
  • Yang W; Institute of Wild Economic Animals and Plants and State Key Laboratory for Molecular Biology of Special Economical Animals, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun, 130112, China.
  • Chang S; Institute of Wild Economic Animals and Plants and State Key Laboratory for Molecular Biology of Special Economical Animals, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun, 130112, China.
  • Zhang J; College of Animal Science and Technology, Jilin Agricultural University, 2888 Xincheng Street, Changchun, 130118, China.
  • Liu Y; College of Animal Science and Technology, Jilin Agricultural University, 2888 Xincheng Street, Changchun, 130118, China.
  • Li Q; College of Animal Science and Technology, Jilin Agricultural University, 2888 Xincheng Street, Changchun, 130118, China.
  • Ge C; College of Vocational and Technical Education, Changchun Sci-Tech University, 1699 Donghua Street, Changchun, 130606, China.
  • Chen G; Key laboratory of Straw Biology and Utilization, The Ministry of Education, Jilin Agricultural University, 2888 Xincheng Street, Changchun, 130118, China.
  • Liu D; Key laboratory of Straw Biology and Utilization, The Ministry of Education, Jilin Agricultural University, 2888 Xincheng Street, Changchun, 130118, China. liudongdong@jlau.edu.cn.
  • Yang F; College of Engineering and Technology, Jilin Agricultural University, 2888 Xincheng Street, Changchun, 130118, China. liudongdong@jlau.edu.cn.
Hereditas ; 157(1): 24, 2020 Jun 26.
Article em En | MEDLINE | ID: mdl-32591015
ABSTRACT

BACKGROUND:

Sika deer is one of the most popular and valued animals in China. However, few studies have been conducted on the microsatellite of Sika deer, which has hampered the progress of genetic selection breeding. To develop and characterize a set of microsatellites for Sika deer which provide helpful information for protection of Sika deer natural resources and effectively increase the yield and quantity of velvet antler.

RESULTS:

We conducted a transcriptome survey of Sika deer using next-generation sequencing technology. One hundred eighty-two thousand two hundred ninety-five microsatellite markers were identified in the transcriptome, 170 of 200 loci were successfully amplified across panels of 140 individuals from Shuangyang Sika deer population. And 29 loci were found to be obvious polymorphic. Number of alleles is from 3 to 14. The expected heterozygosity ranged from 0.3087 to 0.7644. The observed heterozygosity ranged from 0 to 0.7698. The polymorphism information content values of those microsatellites varied ranged from 0.2602 to 0.7507. The marker-trait association was tested for 6 important and kernel characteristics of two-branched velvet antler in Shuangyang Sika deer through one-way analysis of variance. The results showed that marker-trait associations were identified with 8 different markers, especially M009 and M027.

CONCLUSIONS:

This study not only provided a large scale of microsatellites which were valuable for future genetic mapping and trait association in Sika deer, but also offers available information for molecular breeding in Sika deer.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Chifres de Veado / Variação Genética / Cervos / Repetições de Microssatélites / Etiquetas de Sequências Expressas / Estudos de Associação Genética Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Chifres de Veado / Variação Genética / Cervos / Repetições de Microssatélites / Etiquetas de Sequências Expressas / Estudos de Associação Genética Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article