Heat inactivation of serum interferes with the immunoanalysis of antibodies to SARS-CoV-2.
J Clin Lab Anal
; 34(9): e23411, 2020 Sep.
Article
em En
| MEDLINE
| ID: mdl-32594577
ABSTRACT
BACKGROUND:
The detection of serum antibodies to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is emerging as a new tool for the coronavirus disease 2019 (COVID-19) diagnosis. Since many coronaviruses are sensitive to heat, heating inactivation of samples at 56°C prior to testing is considered a possible method to reduce the risk of transmission, but the effect of heating on the measurement of SARS-CoV-2 antibodies is still unclear.METHODS:
By comparing the levels of SARS-CoV-2 antibodies before and after heat inactivation of serum at 56°C for 30 minutes using a quantitative fluorescence immunochromatographic assayRESULTS:
We showed that heat inactivation significantly interferes with the levels of antibodies to SARS-CoV-2. The IgM levels of all the 34 serum samples (100%) from COVID-19 patients decreased by an average level of 53.56%. The IgG levels were decreased in 22 of 34 samples (64.71%) by an average level of 49.54%. Similar changes can also be observed in the non-COVID-19 disease group (n = 9). Of note, 44.12% of the detected IgM levels were dropped below the cutoff value after heating, suggesting heat inactivation can lead to false-negative results of these samples.CONCLUSION:
Our results indicate that heat inactivation of serum at 56°C for 30 minutes interferes with the immunoanalysis of antibodies to SARS-CoV-2. Heat inactivation prior to immunoanalysis is not recommended, and the possibility of false-negative results should be considered if the sample was pre-inactivated by heating.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Pneumonia Viral
/
Imunoensaio
/
Infecções por Coronavirus
/
Soro
/
Betacoronavirus
/
Temperatura Alta
/
Anticorpos Antivirais
Limite:
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article