Branched actin networks are assembled on microtubules by adenomatous polyposis coli for targeted membrane protrusion.
J Cell Biol
; 219(9)2020 09 07.
Article
em En
| MEDLINE
| ID: mdl-32597939
ABSTRACT
Cell migration is driven by pushing and pulling activities of the actin cytoskeleton, but migration directionality is largely controlled by microtubules. This function of microtubules is especially critical for neuron navigation. However, the underlying mechanisms are poorly understood. Here we show that branched actin filament networks, the main pushing machinery in cells, grow directly from microtubule tips toward the leading edge in growth cones of hippocampal neurons. Adenomatous polyposis coli (APC), a protein with both tumor suppressor and cytoskeletal functions, concentrates at the microtubule-branched network interface, whereas APC knockdown nearly eliminates branched actin in growth cones and prevents growth cone recovery after repellent-induced collapse. Conversely, encounters of dynamic APC-positive microtubule tips with the cell edge induce local actin-rich protrusions. Together, we reveal a novel mechanism of cell navigation involving APC-dependent assembly of branched actin networks on microtubule tips.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Actinas
/
Polipose Adenomatosa do Colo
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Proteína da Polipose Adenomatosa do Colo
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Microtúbulos
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article