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Yaravirus: A novel 80-nm virus infecting Acanthamoeba castellanii.
Boratto, Paulo V M; Oliveira, Graziele P; Machado, Talita B; Andrade, Ana Cláudia S P; Baudoin, Jean-Pierre; Klose, Thomas; Schulz, Frederik; Azza, Saïd; Decloquement, Philippe; Chabrière, Eric; Colson, Philippe; Levasseur, Anthony; La Scola, Bernard; Abrahão, Jônatas S.
Afiliação
  • Boratto PVM; Laboratório de Vírus, Instituto de Ciências Biológicas, Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil.
  • Oliveira GP; Microbes, Evolution, Phylogeny and Infection, Aix-Marseille Université UM63, Institut de Recherche pour le Développement 198, Assistance Publique-Hôpitaux de Marseille, 13005 Marseille, France.
  • Machado TB; Laboratório de Vírus, Instituto de Ciências Biológicas, Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil.
  • Andrade ACSP; Microbes, Evolution, Phylogeny and Infection, Aix-Marseille Université UM63, Institut de Recherche pour le Développement 198, Assistance Publique-Hôpitaux de Marseille, 13005 Marseille, France.
  • Baudoin JP; Laboratório de Vírus, Instituto de Ciências Biológicas, Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil.
  • Klose T; Laboratório de Vírus, Instituto de Ciências Biológicas, Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil.
  • Schulz F; Microbes, Evolution, Phylogeny and Infection, Aix-Marseille Université UM63, Institut de Recherche pour le Développement 198, Assistance Publique-Hôpitaux de Marseille, 13005 Marseille, France.
  • Azza S; Institut Hospitalo-Universitaire Méditerranée Infection, Faculté de Médecine, 13005 Marseille, France.
  • Decloquement P; Department of Biological Sciences, Purdue University, West Lafayette, IN 47907.
  • Chabrière E; Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720.
  • Colson P; Microbes, Evolution, Phylogeny and Infection, Aix-Marseille Université UM63, Institut de Recherche pour le Développement 198, Assistance Publique-Hôpitaux de Marseille, 13005 Marseille, France.
  • Levasseur A; Institut Hospitalo-Universitaire Méditerranée Infection, Faculté de Médecine, 13005 Marseille, France.
  • La Scola B; Microbes, Evolution, Phylogeny and Infection, Aix-Marseille Université UM63, Institut de Recherche pour le Développement 198, Assistance Publique-Hôpitaux de Marseille, 13005 Marseille, France.
  • Abrahão JS; Institut Hospitalo-Universitaire Méditerranée Infection, Faculté de Médecine, 13005 Marseille, France.
Proc Natl Acad Sci U S A ; 117(28): 16579-16586, 2020 07 14.
Article em En | MEDLINE | ID: mdl-32601223
ABSTRACT
Here we report the discovery of Yaravirus, a lineage of amoebal virus with a puzzling origin and evolution. Yaravirus presents 80-nm-sized particles and a 44,924-bp dsDNA genome encoding for 74 predicted proteins. Yaravirus genome annotation showed that none of its genes matched with sequences of known organisms at the nucleotide level; at the amino acid level, six predicted proteins had distant matches in the nr database. Complimentary prediction of three-dimensional structures indicated possible function of 17 proteins in total. Furthermore, we were not able to retrieve viral genomes closely related to Yaravirus in 8,535 publicly available metagenomes spanning diverse habitats around the globe. The Yaravirus genome also contained six types of tRNAs that did not match commonly used codons. Proteomics revealed that Yaravirus particles contain 26 viral proteins, one of which potentially representing a divergent major capsid protein (MCP) with a predicted double jelly-roll domain. Structure-guided phylogeny of MCP suggests that Yaravirus groups together with the MCPs of Pleurochrysis endemic viruses. Yaravirus expands our knowledge of the diversity of DNA viruses. The phylogenetic distance between Yaravirus and all other viruses highlights our still preliminary assessment of the genomic diversity of eukaryotic viruses, reinforcing the need for the isolation of new viruses of protists.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Acanthamoeba castellanii / Vírus de DNA Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Acanthamoeba castellanii / Vírus de DNA Idioma: En Ano de publicação: 2020 Tipo de documento: Article