Neonatal Bladder-derived Mesenchymal Stem Cells Ameliorate Bladder Dysfunction in Diabetic Rat Models.
Urol J
; 17(4): 413-421, 2020 06 23.
Article
em En
| MEDLINE
| ID: mdl-32619015
ABSTRACT
PURPOSE:
To evaluate the effect of a new mesenchymal stem cell type derived from the neonatal bladder (nMSC-B) on diabetic bladder dysfunction (DBD). MATERIALS ANDMETHODS:
nMSC-B were harvested from neonatal male Sprague-Dawley rat's bladder and expanded in culture. nMSC-B were transferred to Type-1 diabetic rats which were induced by a single dose 45 mg/kg Streptozocin (STZ). Stem cells were transferred via intraperitoneally (IP) (DM-IP group, n6) and by direct injection to the detrusor (DM-D group, n6) at 12th week following diabetes and compared with Phosphate Buffered Saline (PBS) injected diabetic rats (DM-PBS group, n6) and age-matched PBS injected non-diabetic normal rats (NR-PBS group, n6). All rats were evaluated histopathologically and functionally four weeks after the stem cell treatment.RESULTS:
nMSC-B showed improvement in both voiding function and bladder structure. The maximum voiding pressure (MVP) values in the DM-PBS group were lower compare to DM-IP, DM-D and NR-PBS groups (13.27 ± 0.78 vs 16.27 ± 0.61, 28.59 ± 2.09, 21.54 ± 1.00, respectively, P < .001). There was a significant improvement for MVP values in stem cell-treated groups. Immunohistochemical examination revealed decreased bladder smooth muscle (SM), increased fibrosis and desquamation in urothelia in diabetic groups compared to normal group(P < .001). We detected recovery in the stem cell groups. This recovery was more evident in DM-D group. No statistical difference was observed in SM and fibrosis between DM-D and NR-PBS groups (P = .9).CONCLUSION:
It was shown that nMSCBs provided amelioration of DBD. We think that nMSC-B constitutes an effective treatment method in DBD.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Bexiga Urinária
/
Doenças da Bexiga Urinária
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Complicações do Diabetes
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Células-Tronco Mesenquimais
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article