Imidazole-Based Synthetic Lipidoids for Inâ
Vivo mRNA Delivery into Primary T Lymphocytes.
Angew Chem Int Ed Engl
; 59(45): 20083-20089, 2020 11 02.
Article
em En
| MEDLINE
| ID: mdl-32662132
ABSTRACT
Engineering T lymphocytes is an emerging approach in a variety of biomedical applications. However, delivering large biologics to primary T lymphocytes directly inâ
vivo is technically challenging due to the low transfection efficacy. Herein, we investigated a library of synthetic lipid-like molecules (lipidoids) for their capability of delivering mRNA into primary T lymphocytes both exâ
vivo and inâ
vivo. We initially screened a library with a large structural variety of lipidoids exâ
vivo and identified imidazole-containing lipidoids that are particularly potent in T lymphocytes transfection. We further optimized lipidoid structures by constructing and screening a detailed lipidoid library containing imidazole or imidazole analogues to perform a structure-activity correlation analysis. Using the lead lipidoid as a delivery vehicle for Cre mRNA inâ
vivo through intravenous injection, we achieved 8.2 % gene recombination in mouse T lymphocytes.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
/
Linfócitos T
/
Imidazóis
/
Lipídeos
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article