The effect of Notch signal pathway on PM2.5-induced Muc5ac in Beas-2B cells.
Ecotoxicol Environ Saf
; 203: 110956, 2020 Oct 15.
Article
em En
| MEDLINE
| ID: mdl-32678753
ABSTRACT
BACKGROUND:
Atmospheric pollutants could induced over-expression of Muc5ac, which is a major pathological feature in acute exacerbation of Chronic Obstructive Pulmonary Disease (COPD) and fatal asthma. Notch signaling pathway could promote mucus cell proliferation and mucus secretion. However, the effects of Notch signaling pathway on the airway mucus secretion induced by PM2.5 remain unknown. In this study, we investigated the role of the Notch signaling pathway on Muc5ac by atmospheric PM2.5 in Beas-2B cell.METHODS:
The mRNA and protein levels of the Notch1-4, downstream target gene Hes1 and Muc5ac in the Notch signaling pathway were detected by qPCR and western after Beas-2B cells were exposed to PM2.5 of different concentrations for 12h, 24h, and 48h.RESULTS:
The longer the exposure time and the higher the concentration of PM2.5, the lower the survival rate of Beas-2B cells. The expressions of Hes1 and Muc5ac in mRNA and protein were significantly increased after PM2.5 exposure. Correlation analysis indicated that there was a positive correlation between the expression of Muc5ac and Hes1 in mRNA and protein.CONCLUSION:
Atmospheric PM2.5 can induce the express of Muc5ac, the Notch signaling pathway may be involved in the regulation of Muc5ac by Hes1.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Mucosa Respiratória
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Poluentes Atmosféricos
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Células Epiteliais
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Receptores Notch
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Material Particulado
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Mucina-5AC
Limite:
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article