Effects of bone morphogenetic protein 15 (BMP15) knockdown on porcine testis morphology and spermatogenesis.
Reprod Fertil Dev
; 32(11): 999-1011, 2020 Jul.
Article
em En
| MEDLINE
| ID: mdl-32693912
ABSTRACT
Bone morphogenetic protein 15 (BMP15) is a member of the transforming growth factor-ß (TGFB) superfamily that plays an essential role in mammalian ovary development, oocyte maturation and litter size. However, little is known regarding the expression pattern and biological function of BMP15 in male gonads. In this study we established, for the first time, a transgenic pig model with BMP15 constitutively knocked down by short hairpin (sh) RNA. The transgenic boars were fertile, but sperm viability was decreased. Further analysis of the TGFB/SMAD pathway and markers of reproductive capacity, namely androgen receptor and protamine 2, failed to identify any differentially expressed genes. These results indicate that, in the pig, the biological function of BMP15 in the development of male gonads is not as crucial as in ovary development. However, the role of BMP15 in sperm viability requires further investigation. This study provides new insights into the role of BMP15 in male pig reproduction.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Espermatogênese
/
Espermatozoides
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Testículo
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Proteína Morfogenética Óssea 15
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article