miR-20a-5p promotes pulmonary artery smooth muscle cell proliferation and migration by targeting ABCA1.
J Biochem Mol Toxicol
; 34(12): e22589, 2020 Dec.
Article
em En
| MEDLINE
| ID: mdl-32720422
ABSTRACT
BACKGROUND:
The function of miR-20a-5p in pulmonary artery smooth muscle cells (PASMCs) and the underlying mechanism remains largely unknown.METHODS:
C57BL/6J mice and PASMCs were used for constructing pulmonary artery hypertension (PAH) animal and cell models, respectively. Reverse transcription polymerase chain reaction (RT-PCR) was employed to detect miR-20a-5p and ATP-binding cassette subfamily A member 1 (ABCA1) messenger RNA expression. CCK-8, Transwell, and TUNEL experiments were used to determine PASMCs proliferation, migration, and apoptosis. The relationship between miR-20a-5p and ABCA1 was detected by luciferase reporter experiment, Western blot analysis, and qRT-PCR.RESULTS:
miR-20a-5p was remarkably elevated in PASMCs of PAH mice and human PASMCs treated by hypoxia, while ABCA1 was remarkably decreased. After transfection of miR-20a-5p mimics, PASMCs proliferation and migration were promoted and PASMCs apoptosis was suppressed. ABCA1 was confirmed to be a target of miR-20a-5p and restoration of ABCA1 reversed the function of miR-20a-5p.CONCLUSION:
miR-20a-5p enhances the proliferation and migration of PASMCs to promote the development of PAH via targeting ABCA1.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Artéria Pulmonar
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Movimento Celular
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MicroRNAs
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Proliferação de Células
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Transportador 1 de Cassete de Ligação de ATP
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Músculo Liso Vascular
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article