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Cellular mechanism underlying oxytocin-stimulated Cl- secretion in rat cauda epididymal epithelium.
Gao, Dong-Dong; Wang, Long-Long; Xu, Jia-Wen; Qiu, Zhuo-Er; Zhu, Yun-Xin; Zhang, Yi-Lin; Zhou, Wen-Liang.
Afiliação
  • Gao DD; School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
  • Wang LL; Guangdong Provincial Key Laboratory of Sport and Health Promotion, Scientific Research Center, Guangzhou Sport University, Guangzhou, China.
  • Xu JW; School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
  • Qiu ZE; School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
  • Zhu YX; School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
  • Zhang YL; School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
  • Zhou WL; School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
Am J Physiol Cell Physiol ; 319(4): C630-C640, 2020 10 01.
Article em En | MEDLINE | ID: mdl-32726160
The neurohypophyseal hormone oxytocin (OT) plays critical roles in lactation and parturition, while its function in male reproduction system is largely unknown. This study aims to investigate the effect of OT on regulating transepithelial ion transport in rat cauda epididymal epithelium. With the use of RT-PCR, Western blot, and immunohistochemical analysis, we found that OT receptor (OTR) was expressed and localized at the basal membrane of rat cauda epididymal epithelium. The short-circuit current (Isc) measurement showed that basolateral application of OT to the primary cultured rat cauda epididymal epithelial cells elicited an increase in Isc, which was abrogated by pretreating the epithelial cells with CFTRinh-172, a blocker of cystic fibrosis transmembrane conductance regulator (CFTR). Pretreatment with the prostaglandin H synthase inhibitors indomethacin and piroxicam, or the nonselective antagonists of prostaglandin E2 (PGE2) receptor EP2 or EP4, AH-6809, and AH-23848, significantly attenuated OT-stimulated Isc response. Furthermore, the generation of PGE2 was measured using enzyme-linked immunosorbent assay, demonstrating that OT induced a substantial increase in PGE2 release from primary cultured rat cauda epididymal epithelial cells. In conclusion, activation of OTR by OT triggered PGE2 release, resulting in CFTR-dependent Cl- secretion through paracrine/autocrine pathways in rat cauda epididymal epithelium.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ocitocina / Dinoprostona / Receptores de Ocitocina / Regulador de Condutância Transmembrana em Fibrose Cística Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ocitocina / Dinoprostona / Receptores de Ocitocina / Regulador de Condutância Transmembrana em Fibrose Cística Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article