Cell-specific expression of lung disease risk-related genes in the human small airway epithelium.
Respir Res
; 21(1): 200, 2020 Jul 29.
Article
em En
| MEDLINE
| ID: mdl-32727470
ABSTRACT
BACKGROUND:
The human small airway epithelium (SAE) plays a central role in the early events in the pathogenesis of most inherited and acquired lung disorders. Little is known about the molecular phenotypes of the specific cell populations comprising the SAE in humans, and the contribution of SAE specific cell populations to the risk for lung diseases.METHODS:
Drop-seq single-cell RNA-sequencing was used to characterize the transcriptome of single cells from human SAE of nonsmokers and smokers by bronchoscopic brushing.RESULTS:
Eleven distinct cell populations were identified, including major and rare epithelial cells, and immune/inflammatory cells. There was cell type-specific expression of genes relevant to the risk of the inherited pulmonary disorders, genes associated with risk of chronic obstructive pulmonary disease and idiopathic pulmonary fibrosis and (non-mutated) driver genes for lung cancers. Cigarette smoking significantly altered the cell type-specific transcriptomes and disease risk-related genes.CONCLUSIONS:
This data provides new insights into the possible contribution of specific lung cells to the pathogenesis of lung disorders.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Testes Genéticos
/
Análise de Sequência de RNA
/
Mucosa Respiratória
/
Transcriptoma
/
Fumar Cigarros
/
Pneumopatias
Tipo de estudo:
Diagnostic_studies
/
Etiology_studies
/
Prognostic_studies
/
Risk_factors_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article