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Molecular characterization and expression analysis of the chicken-type and goose-type lysozymes from totoaba (Totoaba macdonaldi).
Moreno-Córdova, Elena N; Islas-Osuna, María A; Contreras-Vergara, Carmen A; López-Zavala, Alonso A; Ruiz-Bustos, Eduardo; Reséndiz-Sandoval, Mónica G; Castillo-Yañez, Francisco J; Criscitiello, Michael F; Arvizu-Flores, Aldo A.
Afiliação
  • Moreno-Córdova EN; Departamento de Ciencias Químico Biológicas, Universidad de Sonora. Blvd. Rosales S/N, Centro. Hermosillo, SON, CP, 83000, Mexico.
  • Islas-Osuna MA; Departamento de Tecnología de Alimentos de Origen Vegetal, Centro de Investigación en Alimentación y Desarrollo, Gustavo Enrique Astiazarán Rosas, N0. 46. Hermosillo, SON, CP, 83304, Mexico.
  • Contreras-Vergara CA; Departamento de Tecnología de Alimentos de Origen Vegetal, Centro de Investigación en Alimentación y Desarrollo, Gustavo Enrique Astiazarán Rosas, N0. 46. Hermosillo, SON, CP, 83304, Mexico.
  • López-Zavala AA; Departamento de Ciencias Químico Biológicas, Universidad de Sonora. Blvd. Rosales S/N, Centro. Hermosillo, SON, CP, 83000, Mexico.
  • Ruiz-Bustos E; Departamento de Ciencias Químico Biológicas, Universidad de Sonora. Blvd. Rosales S/N, Centro. Hermosillo, SON, CP, 83000, Mexico.
  • Reséndiz-Sandoval MG; Laboratorio de Inmunología, Centro de Investigación en Alimentación y Desarrollo, Gustavo Enrique Astiazarán Rosas, N0. 46. Hermosillo, SON, CP, 83304, Mexico.
  • Castillo-Yañez FJ; Departamento de Ciencias Químico Biológicas, Universidad de Sonora. Blvd. Rosales S/N, Centro. Hermosillo, SON, CP, 83000, Mexico.
  • Criscitiello MF; Comparative Immunogenetics Laboratory, Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, 77843, USA; Department of Microbial Pathogenesis and Immunology, College of Medicine, Texas A&M University. Bryan,
  • Arvizu-Flores AA; Departamento de Ciencias Químico Biológicas, Universidad de Sonora. Blvd. Rosales S/N, Centro. Hermosillo, SON, CP, 83000, Mexico. Electronic address: aldo.arvizu@unison.mx.
Dev Comp Immunol ; 113: 103807, 2020 12.
Article em En | MEDLINE | ID: mdl-32735961
Lysozymes play a key role in innate immune response to bacterial pathogens, catalyzing the hydrolysis of the peptidoglycan layer of bacterial cell walls. In this study, the genes encoding the c-type (TmLyzc) and g-type (TmLyzg) lysozymes from Totoaba macdonaldi were cloned and characterized. The cDNA sequences of TmLyzg and TmLyzc were 582 and 432 bp, encoding polypeptides of 193 and 143 amino acids, respectively. Amino acid sequences of these lysozymes shared high identity (60-90%) with their counterparts of other teleosts and showed conserved functional-structural signatures of the lysozyme superfamily. Phylogenetic analysis indicated a close relationship with their vertebrate homologues but distinct evolutionary paths for each lysozyme. Expression analysis by qRT-PCR revealed that TmLyzc was expressed in stomach and pyloric caeca, while TmLyzg was highly expressed in stomach and heart. These results suggest that both lysozymes play important roles in defense of totoaba against bacterial infections or as digestive enzyme.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Muramidase / Proteínas de Peixes / Peixes / Mucosa Gástrica / Antibacterianos / Miocárdio Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Muramidase / Proteínas de Peixes / Peixes / Mucosa Gástrica / Antibacterianos / Miocárdio Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article