An Integrated Microfluidic Probe for Mass Spectrometry Imaging of Biological Samples*.
Angew Chem Int Ed Engl
; 59(50): 22388-22391, 2020 12 07.
Article
em En
| MEDLINE
| ID: mdl-32743957
ABSTRACT
Ambient ionization based on liquid extraction is widely used in mass spectrometry imaging (MSI) of molecules in biological samples. The development of nanospray desorption electrospray ionization (nano-DESI) has enabled the robust imaging of tissue sections with high spatial resolution. However, the fabrication of the nano-DESI probe is challenging, which limits its dissemination to the broader scientific community. Herein, we describe the design and performance of an integrated microfluidic probe (iMFP) for nano-DESI MSI. The glass iMFP, fabricated using photolithography, wet etching, and polishing, shows comparable performance to the capillary-based nano-DESI MSI in terms of stability and sensitivity; a spatial resolution of better than 25â
µm was obtained in these first proof-of-principle experiments. The iMFP is easy to operate and align in front of a mass spectrometer, which will facilitate broader use of liquid-extraction-based MSI in biological research, drug discovery, and clinical studies.
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MEDLINE
Assunto principal:
Útero
/
Técnicas Analíticas Microfluídicas
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article