Knockout of NRAGE promotes autophagy-related gene expression and the periodontitis process in mice.
Oral Dis
; 27(3): 589-599, 2021 Apr.
Article
em En
| MEDLINE
| ID: mdl-32750749
ABSTRACT
BACKGROUND AND OBJECTIVE:
Neurotrophin receptor-interacting MAGE homologue (NRAGE) plays a crucial role in the regulation of bone metabolism. The present study investigated the regulation role of NRAGE on autophagy activation and periodontitis process during experimental periodontitis. MATERIALS ANDMETHODS:
Six-week-old wild-type (WT) and NRAGE-/- mice were randomly divided into three time points in the periodontitis groups (0, 2, and 4 weeks). Histopathological changes were determined using the tooth mobility, hematoxylin and eosin (H&E) staining, and micro-computed tomography (micro-CT). Osteoclasts activation and number were investigated using tartrate-resistant acid phosphatase (TRAP) staining, immunohistochemistry, and real-time quantitative PCR (RT-PCR). The level of autophagy-related gene expression was measured using immunohistochemistry, immunofluorescence, and RT-PCR.RESULTS:
H&E staining and Micro-CT showed that the destruction of the alveolar bone was considerably more severe in the NRAGE-/- group than the WT group after ligation. Tooth mobility in the NRAGE-/- group was obviously higher than that in the WT group. The activation and number of osteoclasts and the level of autophagy-related gene expression in NRAGE-/- group were significantly higher than that in WT group.CONCLUSIONS:
The present study showed that knockout of NRAGE induced autophagy-related gene expression and accelerated the process of periodontitis disease via increasing the activity and differentiation of osteoclast.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Periodontite
/
Perda do Osso Alveolar
Limite:
Animals
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article