Growth of Giant Peptide Vesicles Driven by Compartmentalized Transcription-Translation Activity.
Chemistry
; 26(72): 17356-17360, 2020 Dec 23.
Article
em En
| MEDLINE
| ID: mdl-32777105
ABSTRACT
Compartmentalization and spatial organization of biochemical reactions are essential for the establishment of complex metabolic pathways inside synthetic cells. Phospholipid and fatty acid membranes are the most natural candidates for this purpose, but also polymers have shown great potential as enclosures of artificial cell mimics. Herein, we report on the formation of giant vesicles in a size range of 1â
µm-100â
µm using amphiphilic elastin-like polypeptides. The peptide vesicles can accommodate cell-free gene expression reactions, which is demonstrated by the transcription of a fluorescent RNA aptamer and the production of a fluorescent protein. Importantly, gene expression inside the vesicles leads to a strong growth of their size-up to an order of magnitude in volume in several cases-which is driven by changes in osmotic pressure, resulting in fusion events and uptake of membrane peptides from the environment.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Fosfolipídeos
/
Células Artificiais
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article