DNA flow cytometric values in bladder carcinoma biopsies obtained from fresh and paraffin-embedded material.
APMIS
; 96(1): 25-9, 1988 Jan.
Article
em En
| MEDLINE
| ID: mdl-3278720
ABSTRACT
DNA-histograms were obtained by flow cytometry (FCM) of nuclei prepared from fresh bladder carcinoma biopsies and from 100 micron sections of paraffin-embedded material from the same biopsies. Linear regression analysis of ploidy values from fresh material versus those from paraffin blocks showed excellent correlation (R2 = 0.957). Owing to the high background, the paraffin-embedded material was less suited for analysis of S-phase fraction. It is concluded that DNA FCM of nuclei obtained from paraffin-embedded bladder carcinoma biopsies yields reliable results concerning ploidy, but does not permit evaluation of the S-phase fraction.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Bexiga Urinária
/
DNA de Neoplasias
/
Citometria de Fluxo
Limite:
Humans
Idioma:
En
Ano de publicação:
1988
Tipo de documento:
Article