Methods of Inactivation of SARS-CoV-2 for Downstream Biological Assays.
J Infect Dis
; 222(9): 1462-1467, 2020 10 01.
Article
em En
| MEDLINE
| ID: mdl-32798217
ABSTRACT
The scientific community has responded to the coronavirus disease 2019 (COVID-19) pandemic by rapidly undertaking research to find effective strategies to reduce the burden of this disease. Encouragingly, researchers from a diverse array of fields are collectively working towards this goal. Research with infectious severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is undertaken in high-containment laboratories; however, it is often desirable to work with samples at lower-containment levels. To facilitate the transfer of infectious samples from high-containment laboratories, we have tested methods commonly used to inactivate virus and prepare the sample for additional experiments. Incubation at 80°C, a range of detergents, Trizol reagents, and UV energies were successful at inactivating a high titer of SARS-CoV-2. Methanol and paraformaldehyde incubation of infected cells also inactivated the virus. These protocols can provide a framework for in-house inactivation of SARS-CoV-2 in other laboratories, ensuring the safe use of samples in lower-containment levels.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Inativação de Vírus
/
Betacoronavirus
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article