Application of cold plasma corona discharge in preparation of laccase-based biosensors for dopamine determination.

Laccase-based biosensors were successfully prepared using innovative, cheap, one-step Soft Plasma Polymerization technique by deposition of a bio-recognition layer on glassy carbon electrode and MWCNT (Multi-walled Carbon Nanotubes)-modified glassy carbon electrode. The Soft Plasma Polymerization technique is based on corona discharge of cold atmospheric plasma with close to room temperature. The presented work includes study of biosensor working conditions, optimization of the voltage value applied for corona discharge generation as well as applicability and interference studies for dopamine determination. The biosensor constructed under optimal conditions (corona discharge generated at a voltage of 3 kV and in 30 s time deposition, helium flow rate 10 L/min, laccase solution flow rate 200 µL/min) has two linear ranges from 0.1 µmol/dm3 to 10 µmol/dm3 and from 10 µmol/dm3 to 50 µmol/dm3 with dopamine detection sensitivities of 3.63 µA*dm3/µmol and 1.33 µA*dm3/µmol. Application of the MWCNT interlayer allows the dopamine detection sensitivity to be significantly increased to 22.35 µA* dm3/µmol for a linear range from 0.1 µmol/dm3 to 6 µmol/dm3. Additionally, the studied biosensors have stable and anti-interference ability. Both biosensors were successfully applied for dopamine determination in pharmaceutical preparation.