ß-Lactamase triggered visual detection of bacteria using cephalosporin functionalized biomaterials.
Chem Commun (Camb)
; 56(75): 11098-11101, 2020 Sep 25.
Article
em En
| MEDLINE
| ID: mdl-32812953
ABSTRACT
We report the conjugation of a chromogenic cephalosporin ß-lactamase (ßL) substrate to polymers and integration into biomaterials for facile, visual ßL detection. Identification of these bacterial enzymes, which are a leading cause of antibiotic resistance, is critical in the treatment of infectious diseases. The ßL substrate polymer conjugate undergoes a clear to deep yellow color change upon incubation with common pathogenic Gram-positive and Gram-negative bacteria species. We have demonstrated the feasibility of formulating hydrogels with the ßL substrate covalently tethered to a poly(ethylene glycol) (PEG) polymer matrix, exhibiting a visible color change in the presence of ßLs. This approach has the potential to be used in diagnostic biomaterials for point-of-care detection of ßL-producing bacteria, helping combat the spread of drug resistant microbes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Beta-Lactamases
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Materiais Biocompatíveis
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Compostos Cromogênicos
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Bactérias Gram-Negativas
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Bactérias Gram-Positivas
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Antibacterianos
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article