Construction and screening of a glycosylphosphatidylinositol protein deletion library in Pichia pastoris.

Wang, Pan; Lin, Ying; Zou, Chengjuan; Zhao, Fengguang; Liang, Shuli; Zheng, Suiping; Han, Shuangyan

Wang, Pan; Lin, Ying; Zou, Chengjuan; Zhao, Fengguang; Liang, Shuli; Zheng, Suiping; Han, Shuangyan.

Afiliação

Wang P; Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, Guangdong, China.
Lin Y; Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, Guangdong, China.
Zou C; Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, Guangdong, China.
Zhao F; Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, Guangdong, China.
Liang S; Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, Guangdong, China.
Zheng S; Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, Guangdong, China.
Han S; Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, Guangdong, China. syhan@scut.edu.cn.

BMC Microbiol ; 20(1): 262, 2020 08 24.

Article em En | MEDLINE | ID: mdl-32838766

Assuntos

Proteínas Fúngicas/metabolismo; Proteínas Ligadas por GPI/genética; Técnicas de Inativação de Genes/métodos; Lipase/metabolismo; Pichia/crescimento & desenvolvimento; Fermentação; Proteínas Fúngicas/genética; Deleção de Genes; Biblioteca Gênica; Engenharia Genética; Hidrólise; Lipase/genética; Pichia/genética; Pichia/metabolismo

Palavras-chave

Deletion; GPI protein; Phenotypic screen; Pichia pastoris

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Pichia / Proteínas Fúngicas / Técnicas de Inativação de Genes / Proteínas Ligadas por GPI / Lipase Tipo de estudo: Diagnostic_studies / Prognostic_studies / Screening_studies Idioma: En Ano de publicação: 2020 Tipo de documento: Article

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