Whole blood flow cytometry protocol for the assessment of platelet phenotype, function, and cellular interactions.
Platelets
; 32(6): 786-793, 2021 Aug 18.
Article
em En
| MEDLINE
| ID: mdl-32881599
ABSTRACT
Platelets are a key component of the hemostatic system and their roles in inflammation via interactions with leukocytes have also gained attention in recent years. Changes in platelet phenotype and function can cause bleeding and/or thrombosis and, as such, monitoring platelet-specific changes is crucial to assessing hemostasis in the clinical setting. Currently, available platelet function tests such as platelet aggregometry and thromboelastography require a large volume of blood, which is a major limitation for the pediatric population. Whole blood flow cytometric analysis of platelets is increasingly utilized in recent years, primarily due to the sensitivity of this method, but also because it only requires a small amount of blood with minimal sample manipulation. We have developed a whole blood flow cytometry methodological approach that enables the assessment of platelet phenotype, function, and their interactions with monocytes and neutrophils.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Plaquetas
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Citometria de Fluxo
Limite:
Adolescent
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Child
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Child, preschool
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Female
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Humans
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Infant
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Male
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Newborn
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article