Your browser doesn't support javascript.
loading
Obesity-associated microbiota contributes to mucus layer defects in genetically obese mice.
Schroeder, Bjoern O; Birchenough, George M H; Pradhan, Meenakshi; Nyström, Elisabeth E L; Henricsson, Marcus; Hansson, Gunnar C; Bäckhed, Fredrik.
Afiliação
  • Schroeder BO; Wallenberg Laboratory, Department of Molecular and Clinical Medicine, Institute of Medicine, University of Gothenburg, Gothenburg, Sweden; Laboratory for Molecular Infection Medicine Sweden (MIMS), Department of Molecular Biology, Umeå University, Umeå, Sweden. Electronic address: Bjorn.Schroder@umu
  • Birchenough GMH; Department of Medical BiochemistryInstitute of Biomedicine, University of Gothenburg, Gothenburg, Sweden.
  • Pradhan M; Wallenberg Laboratory, Department of Molecular and Clinical Medicine, Institute of Medicine, University of Gothenburg, Gothenburg, Sweden.
  • Nyström EEL; Department of Medical BiochemistryInstitute of Biomedicine, University of Gothenburg, Gothenburg, Sweden.
  • Henricsson M; Wallenberg Laboratory, Department of Molecular and Clinical Medicine, Institute of Medicine, University of Gothenburg, Gothenburg, Sweden.
  • Hansson GC; Department of Medical BiochemistryInstitute of Biomedicine, University of Gothenburg, Gothenburg, Sweden.
  • Bäckhed F; Wallenberg Laboratory, Department of Molecular and Clinical Medicine, Institute of Medicine, University of Gothenburg, Gothenburg, Sweden; Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark; Department of Clinical Ph
J Biol Chem ; 295(46): 15712-15726, 2020 11 13.
Article em En | MEDLINE | ID: mdl-32900852
The intestinal mucus layer is a physical barrier separating the tremendous number of gut bacteria from the host epithelium. Defects in the mucus layer have been linked to metabolic diseases, but previous studies predominantly investigated mucus function during high-caloric/low-fiber dietary interventions, thus making it difficult to separate effects mediated directly through diet quality from potential obesity-dependent effects. As such, we decided to examine mucus function in mouse models with metabolic disease to distinguish these factors. Here we show that, in contrast to their lean littermates, genetically obese (ob/ob) mice have a defective inner colonic mucus layer that is characterized by increased penetrability and a reduced mucus growth rate. Exploiting the coprophagic behavior of mice, we next co-housed ob/ob and lean mice to investigate if the gut microbiota contributed to these phenotypes. Co-housing rescued the defect of the mucus growth rate, whereas mucus penetrability displayed an intermediate phenotype in both mouse groups. Of note, non-obese diabetic mice with high blood glucose levels displayed a healthy colonic mucus barrier, indicating that the mucus defect is obesity- rather than glucose-mediated. Thus, our data suggest that the gut microbiota community of obesity-prone mice may regulate obesity-associated defects in the colonic mucosal barrier, even in the presence of dietary fiber.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Microbioma Gastrointestinal / Mucosa Intestinal / Obesidade Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Microbioma Gastrointestinal / Mucosa Intestinal / Obesidade Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article