Proteinases released by the parasitic larval stages of Ascaris suum, and their inhibition by antibody.
Mol Biochem Parasitol
; 28(3): 207-16, 1988 Apr.
Article
em En
| MEDLINE
| ID: mdl-3290677
ABSTRACT
The tissue-invasive infective and lung-stage larvae of the nematode Ascaris suum were found to release proteinases during culture in vitro. This activity contained multiple proteolytic enzyme activities, as defined by pH optima, substrate specificities, and inhibitor profiles. Chymotryptic, tryptic collagenolytic and elastolytic activities were produced by both developmental stages, with major pH optima at pH 6 and 9, and there were indications of unusual interactions between the enzymes. The set of proteinases released was found to be specific to each stage of the parasite, although these included some activities which were indistinguishable between the products of the two. The in vitro-released materials of the tissue-parasitic stages of Ascaris are already known to be potently antigenic. Here, we found that this antigenicity was reflected by inhibition of the proteinases of both stages by serum antibody from infected animals. This inactivation of major secreted enzymes of this parasite could presumably contribute to impairment of survival and migratory potential in sensitised hosts.
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Base de dados:
MEDLINE
Assunto principal:
Endopeptidases
/
Inibidores de Proteases
/
Ascaris
/
Anticorpos Anti-Helmínticos
Limite:
Animals
Idioma:
En
Ano de publicação:
1988
Tipo de documento:
Article