[Application of in situ amniocyte culture for prenatal diagnosis].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
; 37(10): 1117-1119, 2020 Oct 10.
Article
em Zh
| MEDLINE
| ID: mdl-32924114
ABSTRACT
OBJECTIVE:
To explore the value of in situ amniocyte culture for prenatal diagnosis.METHODS:
2716 amniotic fluid samples were cultured in situ on slides. After the culture, the slides were stained, photographed and analyzed.RESULTS:
All samples were successfully analyzed, with the success rates for primary culture and subculture being 98.42% and 1.58%, respectively. 224 samples (8.25%) were detected with chromosomal aberrations, which included 125 cases with trisomy 21, 31 with trisomy 18, 3 with trisomy 13, 4 with 45,X, 17 with 47,XXY, 5 with 47,XYY, 1 with 48,XXY,+18, 1 with 48,XXYY, 26 with structural chromosomal aberrations, and 11 with mosaicisms for aneuploidies.CONCLUSION:
In situ amniocyte culture is stable and has a high success rate, and is capable of identifying true and false mosaicisms, which can improve the accuracy of prenatal diagnosis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Diagnóstico Pré-Natal
/
Aberrações Cromossômicas
/
Transtornos Cromossômicos
/
Líquido Amniótico
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Female
/
Humans
/
Pregnancy
Idioma:
Zh
Ano de publicação:
2020
Tipo de documento:
Article