Engineering a methyllysine reader with photoactive amino acid in mammalian cells.
Chem Commun (Camb)
; 56(81): 12210-12213, 2020 Oct 18.
Article
em En
| MEDLINE
| ID: mdl-32926023
ABSTRACT
Methyllysine sites in proteins are recognized by an array of reader domains that mediate protein-protein interactions for controlling cellular processes. Herein, we engineer a chromodomain, an essential methyllysine reader, to carry 4-azido-l-phenylalanine (AzF) via amber suppressor mutagenesis and demonstrate its potential to bind and crosslink methylated proteins in human cells. We further develop a first-of-its kind chromodomain variant bearing two AzF units with enhanced crosslinking potential suitable for profiling the transient methyllysine interactome.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fenilalanina
/
Azidas
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Proteínas Cromossômicas não Histona
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Bioengenharia
/
Lisina
Limite:
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article