Using High-Content Screening to Generate Single-Cell Gene-Corrected Patient-Derived iPS Clones Reveals Excess Alpha-Synuclein with Familial Parkinson's Disease Point Mutation A30P.

Barbuti, Peter; Antony, Paul; Santos, Bruno; Massart, François; Cruciani, Gérald; Dording, Claire; Arias, Jonathan; Schwamborn, Jens; Krüger, Rejko

Barbuti, Peter; Antony, Paul; Santos, Bruno; Massart, François; Cruciani, Gérald; Dording, Claire; Arias, Jonathan; Schwamborn, Jens; Krüger, Rejko.

Afiliação

Barbuti P; Translational Neuroscience, Luxembourg Centre for Systems Biomedicine, University of Luxembourg, L-4362 Luxembourg, Luxembourg.
Antony P; Transversal Translational Medicine, Luxembourg Institute of Health, L-1445 Luxembourg, Luxembourg.
Santos B; Department of Pathology and Cell Biology, Columbia University Irving Medical Center, New York, NY 10032, USA.
Massart F; Translational Neuroscience, Luxembourg Centre for Systems Biomedicine, University of Luxembourg, L-4362 Luxembourg, Luxembourg.
Cruciani G; Translational Neuroscience, Luxembourg Centre for Systems Biomedicine, University of Luxembourg, L-4362 Luxembourg, Luxembourg.
Dording C; Transversal Translational Medicine, Luxembourg Institute of Health, L-1445 Luxembourg, Luxembourg.
Arias J; Translational Neuroscience, Luxembourg Centre for Systems Biomedicine, University of Luxembourg, L-4362 Luxembourg, Luxembourg.
Schwamborn J; Translational Neuroscience, Luxembourg Centre for Systems Biomedicine, University of Luxembourg, L-4362 Luxembourg, Luxembourg.
Krüger R; Translational Neuroscience, Luxembourg Centre for Systems Biomedicine, University of Luxembourg, L-4362 Luxembourg, Luxembourg.

Cells ; 9(9)2020 09 10.

Article em En | MEDLINE | ID: mdl-32927687

Assuntos

Células Clonais/citologia; Células-Tronco Pluripotentes Induzidas/citologia; Doença de Parkinson/genética; alfa-Sinucleína/genética; Diferenciação Celular; Linhagem Celular; Humanos; Doença de Parkinson/patologia

Palavras-chave

A30P; CRISPR-Cas9; Parkinson's disease (PD), patient-derived iPS; SNCA; alpha-synuclein; fluorescent-activated cell sorting (FACS); high-content screening (HCS); isogenic cell lines; single-cell clones

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Doença de Parkinson / Células Clonais / Alfa-Sinucleína / Células-Tronco Pluripotentes Induzidas Tipo de estudo: Diagnostic_studies / Prognostic_studies / Screening_studies Limite: Humans Idioma: En Ano de publicação: 2020 Tipo de documento: Article

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