CA1 Hippocampal Pyramidal Cells in Rats, Resuscitated From 8 Minutes of Ventricular Fibrillation Cardiac Arrest, Recover After 20 Weeks of Survival: A Retrospective Pilot Study.
Shock
; 54(4): 531-538, 2020 10.
Article
em En
| MEDLINE
| ID: mdl-32931694
ABSTRACT
PURPOSE:
The cornu ammonis 1 (CA1) region of the hippocampus is specifically vulnerable to global ischemia. We hypothesized that histopathological outcome in a ventricular fibrillation cardiac arrest (VFCA) rat model depends on the time point of the examination.METHODS:
Male Sprague-Dawley rats were put into VFCA for 8âmin, received chest compressions for 2âmin, and were defibrillated to achieve return of spontaneous circulation. Animals surviving for 80âmin, 14 days and 140 days were compared with controls. Viable neurons were counted in a 500âµm sector of the CA1 region and layer thickness measured. Microglia cells and astrocytes were counted in a 250×300âµm aspect.RESULTS:
Control and 80âmin surviving animals had similar numbers of pyramidal neurons in the CA1 region. In 14 days and 140 days survivors neuron numbers and layer thickness were severely diminished compared with controls (Pâ<â0.001). Two-thirds of the 140 days survivors showed significantly more viable neurons than the last third. Microglia was increased in 14 days survivors compared with controls and 140 days survivors, while astrocytes increased in 14 days and 140 days survivors compared with controls (Pâ<â0.001). 140 days survivors had significantly higher astrocyte counts compared with 14 days survivors.CONCLUSIONS:
The amount and type of brain lesions present after global ischemia depend on the survival time. A consistent reduction in pyramidal cells in the CA1 region was present in all animals 14 days after VFCA, but in two-thirds of animals a repopulation of pyramidal cells seems to have taken place after 140 days.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fibrilação Ventricular
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Região CA1 Hipocampal
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Parada Cardíaca
Tipo de estudo:
Observational_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article