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Xylella fastidiosa subsp. pauca Strains Fb7 and 9a5c from Citrus Display Differential Behavior, Secretome, and Plant Virulence.
de Souza, Jessica Brito; Almeida-Souza, Hebréia Oliveira; Zaini, Paulo Adriano; Alves, Mônica Neli; de Souza, Aline Gomes; Pierry, Paulo Marques; da Silva, Aline Maria; Goulart, Luiz Ricardo; Dandekar, Abhaya M; Nascimento, Rafael.
Afiliação
  • de Souza JB; Institute of Biotechnology, Federal University of Uberlandia, Av. Amazonas, Bloco 2E, Campus Umuarama, 38400-902 Uberlandia MG, Brazil.
  • Almeida-Souza HO; Institute of Biotechnology, Federal University of Uberlandia, Av. Amazonas, Bloco 2E, Campus Umuarama, 38400-902 Uberlandia MG, Brazil.
  • Zaini PA; Department of Plant Sciences, College of Agriculture and Environmental Sciences, University of California, Davis, 1 Shields Ave, Davis, CA 95616, USA.
  • Alves MN; Department of Technology, School of Agricultural and Veterinary Studies, São Paulo State University (FCAV/UNESP), Via de Acesso Prof. Paulo Donato Castellane, 14884-900 Jaboticabal SP, Brazil.
  • de Souza AG; Citriculture Defense Fund (Fundecitrus), Av. Dr. Adhemar Pereira de Barros 201, 14807-040 Araraquara SP, Brazil.
  • Pierry PM; Institute of Biotechnology, Federal University of Uberlandia, Av. Amazonas, Bloco 2E, Campus Umuarama, 38400-902 Uberlandia MG, Brazil.
  • da Silva AM; Department of Biochemistry, Institute of Chemistry, University of São Paulo, Av. Prof. Lineu Prestes 748, 05508-000 São Paulo SP, Brazil.
  • Goulart LR; Department of Biochemistry, Institute of Chemistry, University of São Paulo, Av. Prof. Lineu Prestes 748, 05508-000 São Paulo SP, Brazil.
  • Dandekar AM; Institute of Biotechnology, Federal University of Uberlandia, Av. Amazonas, Bloco 2E, Campus Umuarama, 38400-902 Uberlandia MG, Brazil.
  • Nascimento R; Department of Plant Sciences, College of Agriculture and Environmental Sciences, University of California, Davis, 1 Shields Ave, Davis, CA 95616, USA.
Int J Mol Sci ; 21(18)2020 Sep 15.
Article em En | MEDLINE | ID: mdl-32942709
Xylella fastidiosa colonizes the xylem of various cultivated and native plants worldwide. Citrus production in Brazil has been seriously affected, and major commercial varieties remain susceptible to Citrus Variegated Chlorosis (CVC). Collective cellular behaviors such as biofilm formation influence virulence and insect transmission of X. fastidiosa. The reference strain 9a5c produces a robust biofilm compared to Fb7 that remains mostly planktonic, and both were isolated from symptomatic citrus trees. This work deepens our understanding of these distinct behaviors at the molecular level, by comparing the cellular and secreted proteomes of these two CVC strains. Out of 1017 identified proteins, 128 showed differential abundance between the two strains. Different protein families were represented such as proteases, hemolysin-like proteins, and lipase/esterases, among others. Here we show that the lipase/esterase LesA is among the most abundant secreted proteins of CVC strains as well, and demonstrate its functionality by complementary activity assays. More severe symptoms were observed in Nicotiana tabacum inoculated with strain Fb7 compared to 9a5c. Our results support that systemic symptom development can be accelerated by strains that invest less in biofilm formation and more in plant colonization. This has potential application in modulating the bacterial-plant interaction and reducing disease severity.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Virulência / Citrus / Xylella País como assunto: America do sul / Brasil Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Virulência / Citrus / Xylella País como assunto: America do sul / Brasil Idioma: En Ano de publicação: 2020 Tipo de documento: Article