A Near-Infrared Fluorogenic Pyrrole-Imidazole Polyamide Probe for Live-Cell Imaging of Telomeres.
J Am Chem Soc
; 142(41): 17356-17363, 2020 10 14.
Article
em En
| MEDLINE
| ID: mdl-32955878
ABSTRACT
Telomeres are closely associated with cellular senescence and cancer. Although some techniques have been developed to label telomeres in living cells for study of telomere dynamics, few biocompatible near-infrared probes based on synthetic molecules have been reported. In this study, we developed a near-infrared fluorogenic pyrrole-imidazole polyamide probe (SiR-TTet59B) to visualize telomeres by conjugating a silicon-rhodamine (SiR) fluorophore with a tandem tetramer pyrrole-imidazole polyamide targeting 24 bp in the telomeric double-stranded (ds) DNA. SiR-TTet59B was almost nonfluorescent in water but increased its fluorescence dramatically on binding to telomeric dsDNA. Using a peptide-based delivery reagent, we demonstrated the specific and effective visualization of telomeres in living U2OS cells. Moreover, SiR-TTet59B could be used to observe the dynamic movements of telomeres during interphase and mitosis. This simple imaging method using a synthetic near-infrared probe could be a powerful tool for studies of telomeres and for diagnosis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Pirróis
/
DNA
/
Telômero
/
Corantes Fluorescentes
/
Imidazóis
/
Nylons
Limite:
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article