Protective effects of ß-glucan as adjuvant combined inactivated Vibrio harveyi vaccine in pearl gentian grouper.
Fish Shellfish Immunol
; 106: 1025-1030, 2020 Nov.
Article
em En
| MEDLINE
| ID: mdl-32971269
ABSTRACT
Vaccination is one of the strategies for preventing Vibrio harveyi infection in marine-cultured animals. In this study, we prepared a formalin-killed cells of V. harveyi ZJ0603 vaccine (FKC) combined with ß-glucan to immune pearl gentian grouper. The results indicated that the expression levels of IgM, TNF-α, MHC-Iα, IL-1ß and IL-16 significantly increased in the spleen of the vaccinated fish. Antibody titers, activities of lysozyme and superoxide dismutase were significantly prompted in blood of the vaccinated fish. After 35 d post-vaccination, all fish were challenged intraperitoneally by virulent V. harveyi, and the relative percentage of survival (RPS) of FKC+ß-glucan, FKC, ß-glucan and PBS were 68 ± 5.7%, 55 ± 8.5%, 42 ± 7.5% and 32 ± 6.9%, respectively. These results demonstrated that ß-glucan could be as a potential adjuvant of FKC and provide good protective effect against V. harveyi infection in the pearl gentian grouper culture.
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Base de dados:
MEDLINE
Assunto principal:
Vibrio
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Vibrioses
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Perciformes
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Vacinas Bacterianas
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Vacinas de Produtos Inativados
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Adjuvantes Imunológicos
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Beta-Glucanas
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Doenças dos Peixes
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article