Your browser doesn't support javascript.
loading
UFMylation is associated with LPS-induced inflammatory response in goat endometrial epithelial cells.
Xiao, Jinbang; Liu, Shouqin; Yu, Tong; Zhang, Ruixue; Guo, Xinyan; Jia, Yanni; Shang, Chunmei; Wang, Aihua; Jin, Yaping; Lin, Pengfei.
Afiliação
  • Xiao J; College of Veterinary Medicine, Northwest A&F University, Yangling, China.
  • Liu S; Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, China.
  • Yu T; College of Veterinary Medicine, Northwest A&F University, Yangling, China.
  • Zhang R; Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, China.
  • Guo X; College of Veterinary Medicine, Northwest A&F University, Yangling, China.
  • Jia Y; Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, China.
  • Shang C; College of Veterinary Medicine, Northwest A&F University, Yangling, China.
  • Wang A; Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, China.
  • Jin Y; College of Veterinary Medicine, Northwest A&F University, Yangling, China.
  • Lin P; Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, China.
Reprod Domest Anim ; 55(12): 1725-1734, 2020 Dec.
Article em En | MEDLINE | ID: mdl-32986296
The endometrium plays an important role in the defence against invading pathogens, although the mechanisms are not clear. UFMylation is a recently discovered novel ubiquitination-like modification system that plays a pivotal role in inflammation and the immune response. The purpose of this study was to investigate the effects of UFMylation on lipopolysaccharide (LPS)-induced inflammatory responses in immortalized goat endometrial epithelial cells (gEECs). Ubiquitin-fold modifier conjugating enzyme 1 (UFM1) and DDRGK domain containing 1 (DDRGK1) were mainly localized in the luminal epithelium and glandular epithelium of mouse and goat endometrial tissues. The expression levels of UFM1, ubiquitin-like modifier activating enzyme 5 (UBA5), UFM1 specific ligase 1 (UFL1) and DDRGK1, as key components of the UFMylation system, were significantly activated by 5 µg/mL LPS-induced inflammatory response in gEECs for 6 hr. Meanwhile, the expression levels of interleukin-6 (IL-6) were significantly upregulated, and tumour necrosis factor-α (TNF-α) was significantly down-regulated after overexpression of UFM1 in gEECs. Additionally, we observed UFM1 and DDRGK1 were markedly increased on LPS-stimulated mouse endometritis in vivo. In conclusion, the current study demonstrated that UFMylation was significantly activated by LPS and might be involved in regulating inflammatory response in gEECs.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Endométrio / Ubiquitinação / Inflamação Tipo de estudo: Risk_factors_studies Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Endométrio / Ubiquitinação / Inflamação Tipo de estudo: Risk_factors_studies Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article