Your browser doesn't support javascript.
loading
Transcriptional regulation of prolactin in a euryhaline teleost: Characterisation of gene promoters through in silico and transcriptome analyses.
Seale, Andre P; Malintha, Gardi Hewage Tharindu; Celino-Brady, Fritzie T; Head, Tony; Belcaid, Mahdi; Yamaguchi, Yoko; Lerner, Darren T; Baltzegar, David A; Borski, Russell J; Stoytcheva, Zoia R; Breves, Jason P.
Afiliação
  • Seale AP; Department of Human Nutrition, Food and Animal Sciences, University of Hawai'i at Manoa, Honolulu, HI, USA.
  • Malintha GHT; Department of Human Nutrition, Food and Animal Sciences, University of Hawai'i at Manoa, Honolulu, HI, USA.
  • Celino-Brady FT; Department of Human Nutrition, Food and Animal Sciences, University of Hawai'i at Manoa, Honolulu, HI, USA.
  • Head T; Department of Human Nutrition, Food and Animal Sciences, University of Hawai'i at Manoa, Honolulu, HI, USA.
  • Belcaid M; Hawai'i Institute of Marine Biology, University of Hawai'i at Manoa, Kaneohe, HI, USA.
  • Yamaguchi Y; Institute of Agricultural and Life Sciences, Academic Assembly, Shimane University, Matsue, Japan.
  • Lerner DT; University of Hawai'i Sea Grant College Program, University of Hawai'i at Manoa, Honolulu, HI, USA.
  • Baltzegar DA; Genomic Sciences Laboratory, Office of Research and Innovation, North Carolina State University, Raleigh, NC, USA.
  • Borski RJ; Department of Biological Sciences, North Carolina State University, Raleigh, NC, USA.
  • Stoytcheva ZR; Department of Human Nutrition, Food and Animal Sciences, University of Hawai'i at Manoa, Honolulu, HI, USA.
  • Breves JP; Department of Biology, Skidmore College, Saratoga Springs, NY, USA.
J Neuroendocrinol ; 32(11): e12905, 2020 11.
Article em En | MEDLINE | ID: mdl-32996203
ABSTRACT
The sensitivity of prolactin (Prl) cells of the Mozambique tilapia (Oreochromis mossambicus) pituitary to variations in extracellular osmolality enables investigations into how osmoreception underlies patterns of hormone secretion. Through the actions of their main secretory products, Prl cells play a key role in supporting hydromineral balance of fishes by controlling the major osmoregulatory organs (ie, gill, intestine and kidney). The release of Prl from isolated cells of the rostral pars distalis (RPD) occurs in direct response to physiologically relevant reductions in extracellular osmolality. Although the particular signal transduction pathways that link osmotic conditions to Prl secretion have been identified, the processes that underlie hyposmotic induction of prl gene expression remain unknown. In this short review, we describe two distinct tilapia gene loci that encode Prl177 and Prl188 . From our in silico analyses of prl177 and prl188 promoter regions (approximately 1000 bp) and a transcriptome analysis of RPDs from fresh water (FW)- and seawater (SW)-acclimated tilapia, we propose a working model for how multiple transcription factors link osmoreceptive processes with adaptive patterns of prl177 and prl188 gene expression. We confirmed via RNA-sequencing and a quantitative polymerase chain reaction that multiple transcription factors emerging as predicted regulators of prl gene expression are expressed in the RPD of tilapia. In particular, gene transcripts encoding pou1f1, stat3, creb3l1, pbxip1a and stat1a were highly expressed; creb3l1, pbxip1a and stat1a were elevated in fish acclimated to SW vs FW. Combined, our in silico and transcriptome analyses set a path for resolving how adaptive patterns of Prl secretion are achieved via the integration of osmoreceptive processes with the control of prl gene transcription.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Prolactina / Regulação da Expressão Gênica / Tilápia Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Prolactina / Regulação da Expressão Gênica / Tilápia Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article