Do the human gut metagenomic species possess the minimal set of core functionalities necessary for life?
BMC Genomics
; 21(1): 678, 2020 Sep 30.
Article
em En
| MEDLINE
| ID: mdl-32998695
ABSTRACT
BACKGROUND:
Advances in bioinformatics recently allowed for the recovery of 'metagenomes assembled genomes' from human microbiome studies carried on with shotgun sequencing techniques. Such approach is used as a mean to discover new unclassified metagenomic species, putative biological entities having distinct metabolic traits.RESULTS:
In the present analysis we compare 400 genomes from isolates available on NCBI database and 10,000 human gut metagenomic species, screening all of them for the presence of a minimal set of core functionalities necessary, but not sufficient, for life. As a result, the metagenome-assembled genomes resulted systematically depleted in genes encoding for essential functions apparently needed to support autonomous bacterial life.CONCLUSIONS:
The relevant degree of lacking core functionalities that we observed in metagenome-assembled genomes raises some concerns about the effective completeness of metagenome-assembled genomes, suggesting caution in extrapolating biological information about their metabolic propensity and ecology in a complex environment like the human gastrointestinal tract.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Metagenoma
/
Microbioma Gastrointestinal
/
Genes Bacterianos
Limite:
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article