Investigation of compatibility of severe acute respiratory syndrome coronavirus 2 reverse transcriptase-PCR kits containing different gene targets during coronavirus disease 2019 pandemic.
Future Virol
; 15(8): 515-524, 2020 Aug.
Article
em En
| MEDLINE
| ID: mdl-33005213
ABSTRACT
AIM:
In the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), reverse transcriptase-PCR (RT-PCR) technique is often used. We evaluated the compatibility of SARS-CoV-2 RT-PCR kits containing different gene targets during the pandemic. MATERIALS &METHODS:
Samples were tested by Bio-Speddy® (RdRp gene) and Diagnovital® (RdRp + E genes). The correlation between two assays were determined by Deming regression analysis and chi-square analyses.RESULTS:
Diagnovital PCR kit showed amplification in a narrow Ct range and conveniently sharper exponential amplification curves than Bio-Speedy PCR kit. While the correlation between the findings of the two kits was apparent even with single gene target, this correlation increased when a secondary biomarker was added to the correlation calculations.CONCLUSION:
We have observed high correlation between different PCR kits, however, using different PCR kits during the pandemic may provide a more accurate diagnosis of SARS-CoV-2, since despite correlation there are a number of patients showing contradicting diagnosis.
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MEDLINE
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En
Ano de publicação:
2020
Tipo de documento:
Article