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Gastrointestinal Surgery for Inflammatory Bowel Disease Persistently Lowers Microbiome and Metabolome Diversity.
Fang, Xin; Vázquez-Baeza, Yoshiki; Elijah, Emmanuel; Vargas, Fernando; Ackermann, Gail; Humphrey, Gregory; Lau, Rebecca; Weldon, Kelly C; Sanders, Jon G; Panitchpakdi, Morgan; Carpenter, Carolina; Jarmusch, Alan K; Neill, Jennifer; Miralles, Ara; Dulai, Parambir; Singh, Siddharth; Tsai, Matthew; Swafford, Austin D; Smarr, Larry; Boyle, David L; Palsson, Bernhard O; Chang, John T; Dorrestein, Pieter C; Sandborn, William J; Knight, Rob; Boland, Brigid S.
Afiliação
  • Fang X; Department of Bioengineering University of California, San Diego, CA, USA.
  • Vázquez-Baeza Y; Jacobs School of Engineering University of California, San Diego, CA, USA.
  • Elijah E; Center for Microbiome Innovation University of California, San Diego, CA, USA.
  • Vargas F; Center for Microbiome Innovation University of California, San Diego, CA, USA.
  • Ackermann G; Department of Pharmacology University of California, San Diego, CA, USA.
  • Humphrey G; Department of Pediatrics University of California, San Diego, CA, USA.
  • Lau R; Department of Pediatrics University of California, San Diego, CA, USA.
  • Weldon KC; Department of Cellular and Molecular Medicine University of California, San Diego, CA, USA.
  • Sanders JG; Center for Microbiome Innovation University of California, San Diego, CA, USA.
  • Panitchpakdi M; Collaborative Mass Spectrometry Innovation Center, University of California, San Diego, CA, USA.
  • Carpenter C; Department of Bioengineering University of California, San Diego, CA, USA.
  • Jarmusch AK; Cornell Institute of Host-Microbe Interaction and Disease, Cornell University, Ithaca, NY, USA.
  • Neill J; Department of Pharmacology University of California, San Diego, CA, USA.
  • Miralles A; Center for Microbiome Innovation University of California, San Diego, CA, USA.
  • Dulai P; Department of Pharmacology University of California, San Diego, CA, USA.
  • Singh S; Division of Gastroenterology, Department of Medicine, University of California, San Diego, CA, USA.
  • Tsai M; Division of Gastroenterology, Department of Medicine, University of California, San Diego, CA, USA.
  • Swafford AD; Division of Gastroenterology, Department of Medicine, University of California, San Diego, CA, USA.
  • Smarr L; Division of Gastroenterology, Department of Medicine, University of California, San Diego, CA, USA.
  • Boyle DL; Division of Gastroenterology, Department of Medicine, University of California, San Diego, CA, USA.
  • Palsson BO; Center for Microbiome Innovation University of California, San Diego, CA, USA.
  • Chang JT; Department of Computer Science and Engineering, University of California, San Diego, CA, USA.
  • Dorrestein PC; California Institute for Telecommunications and Information Technology, University of California, San Diego, CA, USA.
  • Sandborn WJ; Division of Rheumatology, Department of Medicine, University of California, San Diego, CA, USA.
  • Knight R; Department of Bioengineering University of California, San Diego, CA, USA.
  • Boland BS; Department of Pediatrics University of California, San Diego, CA, USA.
Inflamm Bowel Dis ; 27(5): 603-616, 2021 04 15.
Article em En | MEDLINE | ID: mdl-33026068
ABSTRACT

BACKGROUND:

Many studies have investigated the role of the microbiome in inflammatory bowel disease (IBD), but few have focused on surgery specifically or its consequences on the metabolome that may differ by surgery type and require longitudinal sampling. Our objective was to characterize and contrast microbiome and metabolome changes after different surgeries for IBD, including ileocolonic resection and colectomy.

METHODS:

The UC San Diego IBD Biobank was used to prospectively collect 332 stool samples from 129 subjects (50 ulcerative colitis; 79 Crohn's disease). Of these, 21 with Crohn's disease had ileocolonic resections, and 17 had colectomies. We used shotgun metagenomics and untargeted liquid chromatography followed by tandem mass spectrometry metabolomics to characterize the microbiomes and metabolomes of these patients up to 24 months after the initial sampling.

RESULTS:

The species diversity and metabolite diversity both differed significantly among groups (species diversity Mann-Whitney U test P value = 7.8e-17; metabolomics, P-value = 0.0043). Escherichia coli in particular expanded dramatically in relative abundance in subjects undergoing surgery. The species profile was better able to classify subjects according to surgery status than the metabolite profile (average precision 0.80 vs 0.68).

CONCLUSIONS:

Intestinal surgeries seem to reduce the diversity of the gut microbiome and metabolome in IBD patients, and these changes may persist. Surgery also further destabilizes the microbiome (but not the metabolome) over time, even relative to the previously established instability in the microbiome of IBD patients. These long-term effects and their consequences for health outcomes need to be studied in prospective longitudinal trials linked to microbiome-involved phenotypes.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Procedimentos Cirúrgicos do Sistema Digestório / Doença de Crohn / Microbioma Gastrointestinal Tipo de estudo: Observational_studies Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Procedimentos Cirúrgicos do Sistema Digestório / Doença de Crohn / Microbioma Gastrointestinal Tipo de estudo: Observational_studies Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article