Lipocalin-type prostaglandin D synthase regulates light-induced phase advance of the central circadian rhythm in mice.
Commun Biol
; 3(1): 557, 2020 10 08.
Article
em En
| MEDLINE
| ID: mdl-33033338
ABSTRACT
We previously showed that mice lacking pituitary adenylate cyclase-activating polypeptide (PACAP) exhibit attenuated light-induced phase shift. To explore the underlying mechanisms, we performed gene expression analysis of laser capture microdissected suprachiasmatic nuclei (SCNs) and found that lipocalin-type prostaglandin (PG) D synthase (L-PGDS) is involved in the impaired response to light stimulation in the late subjective night in PACAP-deficient mice. L-PGDS-deficient mice also showed impaired light-induced phase advance, but normal phase delay and nonvisual light responses. Then, we examined the receptors involved in the response and observed that mice deficient for type 2 PGD2 receptor DP2/CRTH2 (chemoattractant receptor homologous molecule expressed on Th2 cells) show impaired light-induced phase advance. Concordant results were observed using the selective DP2/CRTH2 antagonist CAY10471. These results indicate that L-PGDS is involved in a mechanism of light-induced phase advance via DP2/CRTH2 signaling.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ritmo Circadiano
/
Oxirredutases Intramoleculares
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Lipocalinas
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article