Development of a microchip electrophoresis-based, high-throughput PCR-RFLP method to type Tax 233 variants of bovine leukemia virus in Japan.
Arch Virol
; 165(12): 2961-2966, 2020 Dec.
Article
em En
| MEDLINE
| ID: mdl-33037940
ABSTRACT
Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL). We used microchip electrophoresis in combination with automatic image analysis to develop a novel high-throughput PCR-RFLP to type the gene sequences that encode BLV Tax 233. This method revealed that 233L-Tax is more prevalent than 233P-Tax in cattle in Japan. The proportion infected with BLV carrying the gene encoding 233L-Tax was significantly higher in Holstein cattle than in Japanese Black cattle. Holsteins infected with BLV encoding 233L-Tax had higher proviral loads than did Holsteins infected with BLV encoding 233P-Tax and Japanese Blacks infected with BLV encoding 233L-Tax or 233P-Tax. The novel method developed in this study will be a useful tool for identifying cattle harboring BLV with a higher risk of EBL and viral transmission.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Polimorfismo de Fragmento de Restrição
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Reação em Cadeia da Polimerase
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Produtos do Gene tax
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Vírus da Leucemia Bovina
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Eletroforese em Microchip
Limite:
Animals
País como assunto:
Asia
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article