Bioactive flavonoids from Rubus corchorifolius inhibit α-glucosidase and α-amylase to improve postprandial hyperglycemia.
Food Chem
; 341(Pt 1): 128149, 2021 Mar 30.
Article
em En
| MEDLINE
| ID: mdl-33039745
ABSTRACT
This research established an optimized method for the extraction and enrichment of flavonoids from R. corchorifolius fruit. Under the optimized extraction conditions, 12 flavonoids (1-12) were isolated, of which six (2-4, 9-10, 12) were obtained from R. corchorifolius for the first time. Compound 4 showed significant α-glucosidase (4.96 µM) and α-amylase (8.04 µM) inhibitory effects. Molecular modeling revealed that compound 4 exhibits strong binding with the active sites of α-glucosidase and α-amylase. Lineweaver-Burk plot analysis and surface plasmon resonance revealed the possible dynamic binding mechanism of the flavonoids with α-glucosidase and α-amylase. The enriched flavonoids and compound 4 showed significant hypoglycemic effects in mice administered a high dose of glucose. In this study, a variety of flavonoids with hypoglycemic activity were found for the first time, revealing the rich chemical composition of R. corchorifolius fruit and highlighting the potential value of R. corchorifolius fruit flavonoids as dietary supplements.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Flavonoides
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Alfa-Amilases
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Rubus
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Inibidores de Glicosídeo Hidrolases
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Hiperglicemia
Limite:
Animals
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article