[The Embryo Toxicity Research of Manufacturing Water of Medical Device of in Vitro Fertilization Technology].
Zhongguo Yi Liao Qi Xie Za Zhi
; 44(5): 439-442, 2020 Oct 08.
Article
em Zh
| MEDLINE
| ID: mdl-33047570
ABSTRACT
Water is an important component in liquid medical device products for human assisted reproductive technology. Water traits, conductivity, microbial limits, total organic carbon, easy oxides, heavy metal content, bacterial endotoxin and other indicators have an important impact on sperm, egg and embryo development in vitro, so for such products, the quality of water control is extremely important. The production water for producing such products is generally prepared by MilliQ purification system. In this research, we used four different types of water to fabricate the IVF liquids. It included deionized reverse osmosis water, ultra purified water and ultra purified water without endotoxin or nucleic acid, and compared with tap water. The in vitro rat embryo test system was used to study the embryotoxicity of this four different culture liquid production waters. From the result, the group of the super purified water without endotoxin and nucleic acid has the best result of the embryo formation rate, the number of total cell number and the inner cell number. This study proved the importance of removing endotoxin and nucleic acid from the water used for the preparation of the liquid products for assisted reproduction, and provided the basis for the selection of water quality for the liquid products for assisted reproduction.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Água
/
Fertilização in vitro
/
Contaminação de Equipamentos
/
Técnicas de Reprodução Assistida
/
Desenvolvimento Embrionário
Limite:
Animals
/
Humans
Idioma:
Zh
Ano de publicação:
2020
Tipo de documento:
Article