A post-insertion strategy for surface functionalization of bacterial and mammalian cell-derived extracellular vesicles.
Biochim Biophys Acta Gen Subj
; 1865(4): 129763, 2021 04.
Article
em En
| MEDLINE
| ID: mdl-33065252
ABSTRACT
Extracellular vesicles (EVs) are nanoparticles which are released by cells from all three domains of life Archaea, Bacteria and Eukarya. They can mediate cell-cell communication by transferring cargoes such as proteins and nucleic acids between cells. EVs receive great interest in both academia and industry as they have the potential to be natural drug carriers or vaccine candidates. However, limitations to their clinical translation exist as efficient isolation, loading, labelling and surface-engineering methods are lacking. In this article, we investigate a 'post-insertion' approach, which is commonly used in the functionalization of liposomes in the pharmaceutical field, on two different EV types mammalian cell-derived EVs and bacteria-derived EVs. We aimed to find an easy and flexible approach to functionalize EVs, thereby improving the labelling, isolation, and surface-engineering.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Bactérias
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Imuno-Histoquímica
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Vesículas Extracelulares
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Membrana Externa Bacteriana
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article