Connexin 36 Mediates Orofacial Pain Hypersensitivity Through GluK2 and TRPA1.
Neurosci Bull
; 36(12): 1484-1499, 2020 Dec.
Article
em En
| MEDLINE
| ID: mdl-33067780
ABSTRACT
Trigeminal neuralgia is a debilitating condition, and the pain easily spreads to other parts of the face. Here, we established a mouse model of partial transection of the infraorbital nerve (pT-ION) and found that the Connexin 36 (Cx36) inhibitor mefloquine caused greater alleviation of pT-ION-induced cold allodynia compared to the reduction of mechanical allodynia. Mefloquine reversed the pT-ION-induced upregulation of Cx36, glutamate receptor ionotropic kainate 2 (GluK2), transient receptor potential ankyrin 1 (TRPA1), and phosphorylated extracellular signal regulated kinase (p-ERK) in the trigeminal ganglion. Cold allodynia but not mechanical allodynia induced by pT-ION or by virus-mediated overexpression of Cx36 in the trigeminal ganglion was reversed by the GluK2 antagonist NS102, and knocking down Cx36 expression in Nav1.8-expressing nociceptors by injecting virus into the orofacial skin area of Nav1.8-Cre mice attenuated cold allodynia but not mechanical allodynia. In conclusion, we show that Cx36 contributes greatly to the development of orofacial pain hypersensitivity through GluK2, TRPA1, and p-ERK signaling.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Dor Facial
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Gânglio Trigeminal
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Receptores de Ácido Caínico
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Conexinas
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Canal de Cátion TRPA1
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Hiperalgesia
Limite:
Animals
País como assunto:
Asia
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article