A Role for N6-Methyladenine in DNA Damage Repair.
Trends Biochem Sci
; 46(3): 175-183, 2021 03.
Article
em En
| MEDLINE
| ID: mdl-33077363
ABSTRACT
The leading cause of mutation due to oxidative damage is 8-oxo-2'-deoxyguanosine (8-oxoG) mispairing with adenine (Ade), which can occur in two ways. First, guanine of a GC DNA base pair can be oxidized. If not repaired in time, DNA polymerases can mispair Ade with 8-oxoG in the template. This 8-oxoGA can be repaired by enzymes that remove Ade opposite to template 8-oxoG, or 8-oxoG opposite to Cyt. Second, free 8-oxo-dGTP can be misincorporated by DNA polymerases into DNA opposite template Ade. However, there is no known repair activity that removes 8-oxoG opposite to template Ade. We suggest that a major role of N6-methyladenine in mammalian DNA is minimizing incorporation of 8-oxoG opposite to Ade by DNA polymerases following adduct formation.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Reparo do DNA
/
Guanina
Limite:
Animals
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article